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	Provedor de dados BJMBR (1) Autor Fernandes,K.V.S. (1) Machado,O.L.T. (1) Oliveira,A.E.A. (1) Silva,L.B. (1) Venâncio,T.M. (1) Xavier-Filho,J. (1) Mais... Palavra-chave Cowpea (1) Developing fruits (1) Evolution (1) Insulin (1) Insulin-like (1) Sequence homology (1) Vigna unguiculata (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Ano 2003 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última A protein with amino acid sequence homology to bovine insulin is present in the legume Vigna unguiculata (cowpea) BJMBR Venâncio,T.M.; Oliveira,A.E.A.; Silva,L.B.; Machado,O.L.T.; Fernandes,K.V.S.; Xavier-Filho,J.. Since the discovery of bovine insulin in plants, much effort has been devoted to the characterization of these proteins and elucidation of their functions. We report here the isolation of a protein with similar molecular mass and same amino acid sequence to bovine insulin from developing fruits of cowpea (Vigna unguiculata) genotype Epace 10. Insulin was measured by ELISA using an anti-human insulin antibody and was detected both in empty pods and seed coats but not in the embryo. The highest concentrations (about 0.5 ng/µg of protein) of the protein were detected in seed coats at 16 and 18 days after pollination, and the values were 1.6 to 4.0 times higher than those found for isolated pods tested on any day. N-terminal amino acid sequencing of insulin... Tipo: Info:eu-repo/semantics/article Palavras-chave: Insulin; Insulin-like; Cowpea; Vigna unguiculata; Developing fruits; Sequence homology; Evolution. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900004 Registros recuperados: 1 Primeira ... 1 ... Última

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